anti gli1 rabbit anti human polyclonal antibody  (Bioss)

Journal: Drug Design, Development and Therapy

Article Title: Jujuboside A Regulates Calcium Homeostasis and Structural Plasticity to Alleviate Depression-Like Behavior via Shh Signaling in Immature Neurons

doi: 10.2147/DDDT.S479055

Figure Lengend Snippet: JuA increases the number of immature neurons and activates Shh signaling in the CUMS rats. (A) Representative fluorescence images of BrdU + /DCX + cells; (B) Representative fluorescence images of Shh + , Gli1 + and Gli2 + cells; (C) Number of BrdU + /DCX + cells; (D) Number of Shh + , Gli1 + and Gli2 + cells; (E) Representative immunoblotting bands of Shh, Gli1, Gli2 in each group; (F) Expression levels of Shh, Gli1 and Gli2. n = 3 in each group; the data are presented as the mean ± SD. * p < 0.05 vs the control group. # p < 0.05 vs the CUMS group.

Article Snippet: Next, the sections were incubated with primary antibodies: mouse-anti-BrdU (CST, #5292, 1:1000) following pretreatment, rabbit-anti-Shh (Proteintech, 20697-1-AP, 1:100), rabbit-anti-Gli1 (Bioss, bs-1206R, 1:500), rabbit-anti-Gli2 (Proteintech, 18989-1-AP, 1:500), goat-anti-doublecortin (DCX, Proteintech, 13925-1-AP, 1:100), rabbit-anti-Ca 2+ /calmodulin (CaM)-dependent protein kinase II (CaMKII, Abcam, ab134041, 1:300), rabbit-anti-C-fos proto-oncogene protein (C-fos, Affinity, AF0132, 1:100), anti-PSD-95 (Proteintech, 20665-1-AP, 1:100) and rabbit-anti-Syn (Proteintech, 67864-1-lg, 1:100) at 4°C overnight.

Techniques: Fluorescence, Western Blot, Expressing, Control

Journal: Drug Design, Development and Therapy

Article Title: Jujuboside A Regulates Calcium Homeostasis and Structural Plasticity to Alleviate Depression-Like Behavior via Shh Signaling in Immature Neurons

doi: 10.2147/DDDT.S479055

Figure Lengend Snippet: Knockdown of Shh inhibits the activating effect of JuA on the Shh signaling pathway. (A) Representative fluorescence images of DCX + /Gli1 + cells in rats; (B) Number of DCX + /Gli1 + cells in the vDG; (C) Representative fluorescence images of and DCX + /Gli2 + cells in rats; (D) Number of DCX + /Gli2 + cells in the vDG; (E) Representative immunoblotting bands of Gli1 and Gli2 in each group; (F) Expression levels of Gli1 in the vDG; (G) Expression levels of Gli2 in the vDG. n=3 in each group; the data are presented as the mean ± SD. *p < 0.05 vs the Control group. # p < 0.05 vs the CUMS group. @ p < 0.05 vs the LV-NC group. & p < 0.05 vs the CUMS+JuA group.

Article Snippet: Next, the sections were incubated with primary antibodies: mouse-anti-BrdU (CST, #5292, 1:1000) following pretreatment, rabbit-anti-Shh (Proteintech, 20697-1-AP, 1:100), rabbit-anti-Gli1 (Bioss, bs-1206R, 1:500), rabbit-anti-Gli2 (Proteintech, 18989-1-AP, 1:500), goat-anti-doublecortin (DCX, Proteintech, 13925-1-AP, 1:100), rabbit-anti-Ca 2+ /calmodulin (CaM)-dependent protein kinase II (CaMKII, Abcam, ab134041, 1:300), rabbit-anti-C-fos proto-oncogene protein (C-fos, Affinity, AF0132, 1:100), anti-PSD-95 (Proteintech, 20665-1-AP, 1:100) and rabbit-anti-Syn (Proteintech, 67864-1-lg, 1:100) at 4°C overnight.

Techniques: Knockdown, Fluorescence, Western Blot, Expressing, Control

Journal: Developmental cell

Article Title: Hedgehog signaling activates a mammalian heterochronic gene regulatory network controlling differentiation timing across lineages

doi: 10.1016/j.devcel.2022.08.009

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-GLI1 , Cell Signaling Technology , Cat# 2534S; RRID: AB_2294745.

Techniques: Virus, Subcloning, Recombinant, Modification, Lysis, Western Blot, Bicinchoninic Acid Protein Assay, SYBR Green Assay, Reporter Assay, Control, Transgenic Assay, Software, Staining